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Related post: Washington, DC. 6-5 DEPARTMENT OF HEALTH AND HUMAN SERVICES - PUBLIC HEALTH SERVICE NOTICE OF INTRAMURAL RESEARCH PROJECT PROJECT NUMBER ZOl AI 00485-06 LCMI PERIOD COVERED October Dexamethasone 20mg 1, 1991 to Dexamethasone Pharmacokinetics September 30, 1992 TITLE OF PROJECT (80 characters or less. TiOe must fit on or\e tine between the borders.) T Cell Activation PRINCIPAL INVESTIGATOR (Ust other protassional personnel below the Principal Investigator) (Name, title, laboratory, and institute aftiliation) PI: Ronald H. Schwartz Chief Dexamethasone Ear Drops LCMI, NIAID Others: Scott Umlauf Kurt Brorson Satoru Kitagawa IRTA LCMI, NIAID IRTA LCMI, NIAID Visiting Fellow LCMI, NIAID COOPERATING UNITS (it any) Peter Linsley Bristol-Meyers Squibb LAB/BRANCH Laboratory of Cellular and Molecular Immunology SECTION T Cell Activation Section INSTITUTE AND LOCATION NIAID, NIH, Bethesda, Dexamethasone Inhalation Maryland 20892 TOTAL STAFF Dexamethasone 10 Mg YEARS: 5.5 PROFESSIONAL: 3.5 OTHER: 2 CHECK APPROPRIATE BOX(ES) D (a) Human subjects D (a1) Minors D (a2) Interviews D Dexamethasone Suppresion Test (b) Human tissues B (c) Neither SUMMARY OF WORK (Use standard unreduced type. Do not exceed the space provided.) Studies of T cell activation Dexamethasone Generic Name of normal murine T cell Dexamethasone Gel clones of the ?„! type have shown that two signal?^ are required to stimulate the cells to make interleukin-2 (IL-2) and divide. One signal is given through the antigen-specific receptor that is uniquely expressed on each clone. The other signal is called costimulation and is delivered through a different receptor. Our major objective during the past year was to characterize the costimulatory signal. Other laboratories had identified the B7/BB1 molecule as the inducible ligand on the APC Dexamethasone 20 Mg and CD28 and CTLA-4 on the T cell as potential receptors for costimulation in the production of interleukin-2 (lL-2) . We Dexamethasone 6 Mg explored the role of these molecules in a new assay for costimulation, the induction of competence to respond Dexamethasone 0.75 Mg to interleukin-4 (IL-4) . Murine T^l clones do not respond directly to IL-4. They first must be activated by stimulating them with antigens and APC. After 24 hr. the activated T Dexamethasone Supression Test cells then proliferate in response to IL-4. We demonstrated that costimulation is required during the induction phase in order to make the clones competent to proliferate in response to IL-4. Although supplying costimulation also led to the production of IL-2, stimulation with this Dexamethasone Pharmacology lymphokine was Dexamethasone Crh Test not the only signal required to gain competence, because addition of IL-2 to a T cell receptor occupancy signal only allowed the cells to become partially competent to respond to IL-4 . We concluded from these experiments that the costimulatory signal contributes directly to the attainment of full competence Dexamethasone Syrup to respond to IL-4. To explore the molecules involved in this costimulation, we used a recombinant fusion protein between CTLA4 and a human Ig immunoglobulin constant region. This molecule reacts with B7/BB1 expressed Dexamethasone Sp on activated antigen-presenting cells. We showed that Dexamethasone Acne CTLA4Ig could inhibit proliferation and IL-2 production of both ThI and T„0 murine clones but not IL-4 production by the latter. In addition it inhibited the ability of T„l clones to gain competence to respond to IL-4. We concluded from these experiments that B7/BB1 is the critical ligand involved in costimulation for both IL-2 production and the gaining of competence to respond to IL-4. PHy e04U (Hev. Dexamethasone 40 Mg Buy Dexamethasone Online 5,52) 6-6 DEPARTMENT OF HEALTH AND HUMAN SERVICES - PUBLIC HEALTH SERVICE NOTICE OF INTRAMURAL RESEARCH PROJECT PROJECT NUMBER ZOl AI 00613-02 LCMI PERIOD COVERED October 1, 1991 to September 30, 1992 TITLE OF PROJECT (80 characters or lass. TWe must fit or) one line between the borders.) T Cell Subsets PRINCIPAL INVESTIGATOR (List other professional personnel below the Principal Investigator.) (Name, title, laboralory, and Irtstitute affllialion) PI: Ronald H. Schwartz Chief LCMI, NIAID Others: Bart Beverly Staff Fellow LCMI, NIAID Albert Bendelac Visiting Associate LCMI, NIAID Delanie Cassell IRTA LCMI, NIAID COOPERATING UNITS (if any) David Ailing LCI, NIAID LAB/BRANCH Laboratory of Cellular and Molecular Immunology SECTION T Cell Activation Section INSTITUTE AND LOCATION NIAID, Dexamethasone 4mg Tablet NIH, Bethesda, Maryland 20892 TOTAL STAFF YEARS: 5.5 PROFESSIONAL: 3.5 OTHER:
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