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Related post: Z01 Al Project Number Summary 00480-07 00488-06 00492-06 00552-04 00553-04 00554-04 LABORATORY OF VECTORS AND PATHOGENS Rocky Mountain Laboratories Hamilton, Montana 1992 Annual Report Table of Contents Pathogen-arthropod Interactions of Vector-borne Diseases Affecting Public Health - Schwan Uitrastructural Analysis of Antigenic Determinants In Pathogens - Garon Molecular Basis for Infection by Borrelia burgdorferi - Schwan Immunopathobiology of Bacterial Toxins - Cieplak Molecular Biology and Immunology of Pathogenic Campylobacter spp. - Cieplak Structural Characterization of Microbial Genes and Nucleic Acid Dexamethasone Iv Molecules - Garon Page 20-1 20-8 20-9 20-10 20-11 20-12 20-13 ANNUAL REPORT LABORATORY OF VECTORS AND PATHOGENS HAMILTON, MONTANA NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES OCTOBER Dexamethasone 0.5 1, 1991, TO SEPTEMBER 30, 1992 Members of the Laboratory of Vectors and Pattiogens have continued their molecular dissection of important human pathogens during the past year. This multidisciplinary approach to microbial pathogenesis combines the fields of biochemistry, immunology, electron microscopy, medical entomology and molecular biology into a cohesive unit with strong interaction among its own members and with scientific units both inside and outside of the NIH Dexamethasone 4 Mg system. This interaction together with an active seminar and guest researcher program insures that the best experimental approaches available are rapidly incorporated into the research programs of the laboratory. Since Lyme borreliosis is now the most common arthropod-borne disease in the United States, the Laboratory has made a major commitment of resources to the development of improved diagnostics and to a detailed understanding of how Borrelia burgdorferi produces its long list of both acute and chronic clinical features. The Laboratory of Vectors and Pathogens functions in three major experimental groups. Dr. Claude F. Garon sen/es as Laboratory Chief. The Arthropod-borne Diseases Section, under the direction of Dr. Tom G. Schwan, is involved is numerous investigations relating to the molecular biology, infectivity, pathogenicity, antigenicity, and immunogenicity of B. Oral Dexamethasone burgdorferi. The history of Lyme serology dates back only 10 years to 1982 when the first test, an indirect fluorescent antibody (IFA), was reported using the newly discovered spirochete. However, the lack Dexamethasone Table of specificity in the IFA and ELISA using whole B. burgdorferi cells has prompted them to search for components of the bacterium that can be used as diagnostic antigens that will improve the specificity and sensitivity Dexamethasone Ophthalmic Drops at detecting antibodies to B. burgdorferi. Previously, the Dexamethasone 5 Mg group cloned and expressed a 39 kDa protein of the Lyme spirochete and demonstrated its reactivity with serum from Lyme patients. Recently, additional bacteria were examined to further clarify the specificity of Neomycin Polymyxin Dexamethasone this protein to only Lyme spirochetes. Tobramycin Dexamethasone Ophthalmic SDS-PAGE and immunoblotting were used to examine bacteria and convalescent human serum samples. Five serovars of Leptospira interrogans, Leptospira biflexa, Leptonema illini, and Ricl
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