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Related post: established, cell hybridization and transformation with DNA, many questions pertaining to the control of gene expression and of cellular differentiation became accessible. It is clear, however, that to exploit these opportunities broadly, resources in the form of a broad range of established cell lines with defined function will be needed. 4-10 3. The Serum Bank The mission of the Serum Bank has been to provide high quality sera and cells for tissue typing to support research in immunogenetics and transplantation immunology. The Duphalac Dosage bank therefore acquired, through purchase and donations, sera recognizing antigens encoded by the A, B, C, and DR loci of the human major histocompatibility complex, and typing cells homozygous at the D locus, evaluated these reagents, and distributed them (the sera in bulk form and on the NIH typing tray) to basic and clinical investigators. During the past year the Serum Bank underwent major administrative and conceptual restructuring. Much of the routine activity of monitoring the flow of reagents in and out of the Bank has been automated. Analysis of the evaluations of the tests performed by reference laboratories to determine the quality of the sera submitted has been recaptured. Firm policies Lactulose Duphalac regarding the distribution of sera, restricting the availability of the rare specifities and reemphasizing that the Bank exists to support research, were issued. A revised catalog supplement describing the sera in the Bank and specifying the restrictions on Duphalac Lactulose their distribution was published. As a consequence confidence in the Bank was restored, attested to by a great resurgence of offers of sera, including those specific for rare antigens. This permitted the addition of 42 liters of sera to the Bank's inventory, compared to an outflow of 13 liters, the average distribution in recent years, thus rebuilding the stocks and permitting the removal of several sera from the restricted category. The redesign of the typing tray represents a major conceptual innovation. The standard tray had been replaced by a Basic and an Extended Tray system. The Basic Tray recognizes the commonly encountered antigens and the cross-reactive groups of the A and B loci. It should suffice in the great majority of typings for transplantation, especially those involving living related donor grafts, and will be available for research on an unrestricted basis. The Basic Tray was designed to achieve two purposes. The first, just stated, will result in the more economical use of rare sera and therefore Duphalac Syrup will enable the Bank to make the trays more broadly available (heretofore they were distributed solely to support human organ transplantation). The second was to enable private organizations to produce it by including on it only the more readily available antigen specificities, and so to permit the Bank to, ultimately, cease producing it Itself. This objective was announced to the tissue typing community and to organizations with a capability in this area. Several are prepared to accept responsibility for the Basic Tray, in toto, but further progress towards this end awaits delicensing of the sera and their regulation as "medical devices" by the Food and Drug Administration. 4-11 Recognizing, however, that on occasion, the Basic Tray will prove inadequate to ascertain an individual's transplantation antigens and that the rare sera needed to achieve this are not and will not be readily available, the Bank will continue to produce the companion Extended Tray. This tray carries sera for rare specificities and splits at the Syrup Duphalac A and B loci and sera recognizing antigens coded by the C locus. This tray is produced in limited quantities and its distribution will be more closely controlled. The Bank has also undertaken to produce trays to serve specialized needs. The first of these is the DR locus tray. A matter currently under intense study is the utility of matching at the DR locus in, especially, renal transplantation. The DR tray should provide a broadly available standard set of high-quality reagents to facilitate research on this question. Additional trays are under consideration but their production hinges on the acquisition of appropriate sera. For example, it is generally accepted that blacks frequently Buy Duphalac cannot be tissue typed satisfactorily with the commonly available sera obtained, by and large, from non-black women (pregnancy serves to immunize) . A workshop to define the magnitude of the problem and to indicate promising approaches is scheduled for September 1980. It is expected that it will be followed by a Duphalac Buy planned program for the acquisition and testing of sera recognizing black antigens Duphalac Dose and finally by a tray that will carry them and will be used to type blacks. Reagents of another type are potentially of great use in human histocompatibility testing but sufficient experience with them is not yet available. These are the monoclonal antibodies produced by mouse hybridomas. To evaluate them the Branch has organized a workshop. The first step, to be taken in October 1980, will be to examine the various techniques that may be useful in their application as typing reagents and to select the most practical one. In the next stage, the 50 or so monoclonal antibodies recognizing human polymorphic cell surface antigens will be prescreened and then distributed to laboratories possessing well-studied families and broad population panels.
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